Western Blot analysis of Rat ovary lysate (20ug/lane) using I7661-16M ( 1:2000) Secondary: IgG (H+L) (HRP) goat anti-rabbit (1:10,000) Predicted band size: 28kD Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A549 cells stained with I7661-16M (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% BSA to block non-specific protein-protein interactions followed by I7661-16M (1:25) for 60 min at 37ºC. The secondary antibody used was goat-anti-rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1:200) for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.